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martin spiess  (Addgene inc)


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    Structured Review

    Addgene inc martin spiess
    Martin Spiess, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/martin+spiess/pET24a-VHH-tev-mCherry+(Plasmid+%23117752)/pm40204056-261-12-15
    Average 93 stars, based on 1 article reviews
    martin spiess - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cloning:

    Article Title: Exploiting Ligand-binding Domain Dimerization for Development of Novel Androgen Receptor Inhibitors
    Article Snippet: AR-LBD expression and purification A pET expression plasmid containing Thioredoxine-His-Thrombin-TEV- AR LBD (663–919)- GSGSGS-Avi-tag (GLNDIFEAQKIEWHE) was cotransformed with pSAM-2xflag-BirA in in BL21 (DE3)-RILP codon plus competent cells (Agilent Technologies). .. This pSAM-2xflag-BirA was created by integrating the BirA cassette from pET21d-myc-BirA into pSAM using specific primers that contain a double flag-tag and sufficient overhang to perform infusion cloning. pET21d-myc-BirA was a gift from Martin Spiess (Addgene plasmid # 109424; http://n2t.net/addgene:109424; RRID: Addgene_109424; ref. 28) and pSAM was a gift from Jumi Shin (Addgene plasmid # 45174; http://n2t.net/addgene:45174; RRID: Addgene_45174; ref. 29). .. Protein expression is induced with 1mmol/L IPTG and in presence of 20 mmol/L DHT and 125 mmol/L biotin (Sigma-Aldrich) at 15 C for 18 hours.

    Plasmid Preparation:

    Article Title: Exploiting Ligand-binding Domain Dimerization for Development of Novel Androgen Receptor Inhibitors
    Article Snippet: AR-LBD expression and purification A pET expression plasmid containing Thioredoxine-His-Thrombin-TEV- AR LBD (663–919)- GSGSGS-Avi-tag (GLNDIFEAQKIEWHE) was cotransformed with pSAM-2xflag-BirA in in BL21 (DE3)-RILP codon plus competent cells (Agilent Technologies). .. This pSAM-2xflag-BirA was created by integrating the BirA cassette from pET21d-myc-BirA into pSAM using specific primers that contain a double flag-tag and sufficient overhang to perform infusion cloning. pET21d-myc-BirA was a gift from Martin Spiess (Addgene plasmid # 109424; http://n2t.net/addgene:109424; RRID: Addgene_109424; ref. 28) and pSAM was a gift from Jumi Shin (Addgene plasmid # 45174; http://n2t.net/addgene:45174; RRID: Addgene_45174; ref. 29). .. Protein expression is induced with 1mmol/L IPTG and in presence of 20 mmol/L DHT and 125 mmol/L biotin (Sigma-Aldrich) at 15 C for 18 hours.

    Article Title: Characterization of Biomolecular Interactions by Biophysical Methods.
    Article Snippet: .. Purification of the anti-GFP nanobody (GFP-enhancer): Anti-GFP nanobody was a gift from Martin Spiess (“pET24a-VHH-TEV-mCherry “Addgene plasmid #117752) and protein was purified following D20 ̊C = DT,B( ηT,B η20 ̊C )Eq. ..

    Purification:

    Article Title: Characterization of Biomolecular Interactions by Biophysical Methods.
    Article Snippet: .. Purification of the anti-GFP nanobody (GFP-enhancer): Anti-GFP nanobody was a gift from Martin Spiess (“pET24a-VHH-TEV-mCherry “Addgene plasmid #117752) and protein was purified following D20 ̊C = DT,B( ηT,B η20 ̊C )Eq. ..



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    martin spiess  (Addgene inc)
    93
    Addgene inc martin spiess
    Martin Spiess, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/martin+spiess/pET24a-VHH-tev-mCherry+(Plasmid+%23117752)/pm40204056-261-12-15
    Average 93 stars, based on 1 article reviews
    martin spiess - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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